Technology Offers

Innovative coating for implants for improved ingrowth into the bone

Abstract

Scientists at the Universities of Stuttgart and Constance have developed a technology which should significantly improve ingrowth of implants into the surrounding tissue and at the same time ensure long-term stability.

A new type of implant coating created of a non-water-soluble, functionalized extracellular matrix (FECM) is achieved by means of metabolic oligosaccharide engineering. The coating improves the cell-material interaction at the interface between tissue and biomaterials (e. g. titanium). Since the patient's own cells can be used to produce the FECM, no immunogenicity of the implant coating is to be feared.

Background

In a human tissue, the native human extracellular matrix (ECM) forms the natural environment of the cells. It consists of a complex network of various organic molecules and is, among other functions, involved in the construction and reconstruction of tissues. In the present technology which is designed to improve the cell-material interaction at the interface between tissues and artificial materials, in particular for implants, the high biological activity is used to improve the biocompatibility of the implant. The ECM is modified by functional groups in order to generate covalent (strong) chemical bondings to a complementary functionalized material surface. That way it is possible to generate an implant coating that is much more complex and stable in comparison to conventional biological coatings with a single biopolymer. Moreover, it improves ingrowth of the implant into the surrounding tissue due to the high biological activity of the complex ECM used. 

Problem

Implants for bone reconstruction are often made of metals such as titanium. These implant materials are perceived as a foreign body for the organism. Despite a good overall biocompatibility of the material, its integration (e.g. into the bones of the patient) is still problematic today. In order to improve integration into natural tissue, varied methods were developed to create surface structures and properties of metallic implants that are as close to the natural bone structure ("bone mimic") as possible. Examples of such methods include the design of special surface modifications or the use of coatings that imitate bone properties such as hydroxyapatite coatings. However, all these methods have disadvantages and do not lead to completely satisfactory results. Until now, it has not been possible to provide coatings with a complex biological material like ECM, in particular in terms of sufficient durability on a planar or smooth carrier material. Conventional coatings with a complex ECM have so far been based on physisorption, i.e. the attachment of biomolecules by physical interaction with the surface, which often could not generate sufficient stability.

Solution

The Universities of Stuttgart and Constance have now developed an innovative method which allows functional chemical groups to be incorporated in the complex ECM in a natural manner by means of metabolic oligosaccharide engineering. These groups can then form stable, covalent bonds to complementary functionalized surfaces through bioorthogonal ligation reactions (so-called click reactions). This allows the creation of a non-water-soluble, click-functional ECM (clickECM) coating, which, apart from the functional groups, does not differ from the naturally occurring matrices. The material to be coated, such as titanium or other artificial materials can be functionalized with the suitable click groups by means of plasma technology or wet-chemical methods. Since the donor's own cells are used to produce the clickECM, no immunogenicity of the implant coating is to be feared.

Graphic representation of coating process
By adding functionalized monosaccharides to the cell culture medium (A) an FECM (B) is created which can be isolated and purified. Titanium implants such as hip prosthesis (D) can be coated with this FECM via a covalent chemical reaction (F) after a wet-chemical modification (E).
Representation of cell adhesion on differently coated surfaces

Advantages

Coating of an implant (carrier) using clickECM:

  • similar to the biological tissue
  • enables stable coatings under physiological conditions
  • easy production and purification methods
  • personalized coating: improved tolerability as the material is made of the donor's own cells
  • can be provided in a sterile manner
  • can be produced inexpensively

Application

Coating for implants

Exposé
Contact
Dr. Frank Schlotter
TLB GmbH
Ettlinger Straße 25
76137 Karlsruhe | Germany
Phone +49 721-79004-0
schlotter(at)tlb.de | www.tlb.de
Development Status
Late basic research / TRL2
Patent Situation
DE 102014222898 A1 pending
PCT pending
Reference ID
14/058TLB
Service
Technologie-Lizenz-Büro GmbH is responsible for the exploitation of this technology. It offers companies the possibility of cooperation and assists them in obtaining licenses.